Since the launch of this blog, The Genetic Link, in 2009, we have published numerous articles, interviews, technical information and infographics to share what we know about DNA from saliva. Saliva is the human body’s most accessible bio-fluid but, after 7 years, we continue to be surprised at how often we hear wrong perceptions about this robust and easy to access sample type. It’s time to set the record straight on DNA from saliva once and for all. Are you ready? Here are 8 facts about DNA from saliva that most people don’t know.Read More
DNA Genotek's Sample Collection Blog
Scott Rabuka is a senior product manager at DNA Genotek.
We are always looking for ways to help our customers get the most out of Oragene. We learn daily about customers who are using Oragene in ways we never expected, and when they share what they’ve learned with us, it means we can pass our newfound knowledge along to others looking for solutions to similar problems. We decided to try some new approaches to build even more collaboration and knowledge sharing between our customers.
Collecting saliva DNA is more effective and less costly for genetic research, a study suggests. The study, titled Specimen Collection within the CRN: A critical appraisal, was produced by The CRN Pharmacovigilance Project Genomics Working Group. The overall goal of the genomics working group was to explore issues surrounding the collection and use of biological specimens at the participating CRN sites. This report is one of the most comprehensive analyses by an organization on the cost-effectiveness of blood and saliva sample collection for DNA applications. The saliva collection analysis was based on the Oragene kit.
DNA from Oragene/saliva samples continues to gain acceptance as a suitable sample input for next generation sequencing technologies (NGS) from targeted to exome to whole genome sequencing. The latest development in this body of evidence is the recent announcement by Complete Genomics that they will now accept DNA from Oragene/saliva samples for whole genome sequencing.
I am pleased to announce the launch of ORAcollect•DNA (OCR-100), the newest product in the DNA Genotek family. ORAcollect•DNA is a sample collection kit that uses the same underlying technology as Oragene•DNA, but with a different collection method. The fundamental difference between Oragene and ORAcollect•DNA is the way the sample is collected. Instead of spitting into an Oragene kit, the patient/donor uses the integrated sponge to collect the sample and then invert the sponge and secure it in the collection tube.
A few weeks ago, I posted an article about best practices for long term storage of unpurified Oragene•DNA samples on The Genetic Link. The response to the article was positive so I'm following up with today's story about long term storage of DNA purified from Oragene•DNA samples. The preservation and storage of DNA is an important consideration for molecular epidemiology and population studies. As the makers of the Oragene•DNA family of products, we have prepared these recommendations for the long-term storage of DNA purified from saliva samples collected with Oragene to help our customers achieve optimal results.
Oragene is well known for a number of characteristics - ease of use, non-invasive collection, high quality and quantity DNA and, of course, long term storage at ambient temperature. It's often difficult for researchers and clinicians to believe that storage at ambient temperature is possible for DNA samples. We are frequently asked about this specification of our product. However, studies using Oragene prove that customers can rely on the ability to store Oragene/saliva samples at ambient temperatures when collecting samples in remote locations, via the mail, or for event-based recruitment. The figure to the right shows an agarose gel electrophoresis of DNA extracted from Oragene/saliva samples stored at room temperature for years.
At DNA Genotek, we understand the importance of high quality DNA samples to support our customers' research and clinical studies. As a result, we work with our customers to ensure the results they obtain with our products are optimized. Implementing proven techniques and procedures for maximizing the DNA yield from Oragene/saliva samples can increase the yield and improve the efficiency of laboratory processes and quality of downstream results. Recently, we created a list of 'best practices' to ensure that you achieve the maximum possible yield from Oragene/saliva samples. We want to share this list with you.