Mike Tayeb is the Technical Support Manager at DNA Genotek Inc.
DNA Genotek's Sample Collection Blog
We are very excited to tell you about a new award from DNA Genotek. Today, we introduced the GenoFIND Genomic Services Award, which is designed to facilitate analysis of nucleic acid samples. The award will accelerate genetic discoveries by providing $10,000 US in GenoFIND services to the selected winner. GenoFIND is a compilation of commercially available genomic services that include: nucleic acid extraction, SNP genotyping, PCR-based assays, whole genome and targeted sequencing, array-based SNP and expression profiling, custom assay development and bioinformatic analysis.
If there's one thing that makes us excited (other than saliva DNA samples, of course), it's seeing samples that are optimized to ensure the best possible results. Great DNA yields from saliva enables our customers to do more with their samples and non-invasive collection options provide researchers and participants with a collection method they actually like. But to get so excited about great DNA samples means there's another end of the spectrum. Therefore, we thought it would be helpful to highlight proven tips and tricks for maximizing your samples.
We recently published an article highlighting how to choose the best method for low-throughput DNA extraction for Oragene samples. Today, we examine various options for high-throughput DNA extraction. High-throughput DNA extraction uses an automated, robotic system to provide researchers and clinicians with high quality genomic DNA. The main advantages of this method are less hands-on time, higher consistency and reduced probability for manual errors. High-throughput extraction is generally preferred when there are a large number of samples to be processed, however whether processing a small or a very large number of samples, automation is a great way to gain efficiencies and consistency in results.
DNA is extracted from human saliva for a variety of reasons including molecular or forensic analysis. There are many benefits associated with collecting DNA from saliva with Oragene® kits such as non-invasive collection, high quality DNA and long-term storage at ambient temperatures. Once collection of the samples is complete, DNA extraction becomes a key stage in the processing of samples within most genomic laboratories. Challenges with DNA extraction can have an impact on downstream assay performance and even if you’re not having challenges, it’s always nice to find some information that might improve your technique. In this article, we focus on low-throughput DNA extraction methods from samples collected with Oragene. Low-throughput processing typically refers to manual processing of samples although there are also several automated platforms that process small batches of samples.
We are so pleased to let you know that we have released our first video protocol for the extraction of DNA from the Oragene and ORAcollect families of collection kits. We know how much our customers rely on our written protocols for the purification of DNA from saliva with our products, so we wanted to offer you an additional tool as you begin processing your samples. This first video is for the DNA Genotek protocol titled Laboratory protocol for manual purification of DNA from 0.5mL of sample (protocol # PD-PR-006). This is our most popular protocol so we decided to make this one available in video format first.