By Ellen MacLean on Thu, Dec 10, 2009 @ 15:12 PM
People often think of genetic research and testing as a solely human pursuit but scientists and clinicians often study animals and plants as well. Genetic researchers and clinicians study models of animal disease in animals as well as human diseases with animal models.
One such researcher is Dr. Katherine Mitsouras, PhD, of the Western University of Health Sciences. She recently published new research on the use of saliva as an alternative source of high yield canine DNA for genotyping studies. Animal researchers and pet owners alike will be pleased with the results of this research.
According to Dr. Mitsouras: "The domestic dog presents an attractive model system for the study of the genetic basis of disease. The development of resources such as the canine genome sequence and SNP genotyping platforms has allowed for the implementation of canine genetic studies. Successful implementation of such studies depends not only on the quality of individual DNA samples, but also on the number of samples obtained."
The objective of the study was to compare the yield and quality of DNA obtained from matched buccal swab, blood and Oragene•ANIMAL saliva samples. The performance of these samples was assessed for use on PCR-based downstream applications.
The study determined that DNA yields from canine saliva are higher than those from blood or buccal swabs (see Table 1 for details). The quality of DNA extracted from saliva is sufficient for successful amplification of a 1.1-kb fragment and for accurate SNP genotyping by PCR-RFLP. Therefore, saliva presents a non-invasive alternative source of high quantities of canine genomic DNA suitable for genotyping studies.
For researchers, veterinarians and pet owners, this is exciting news. In short, using Oragene•ANIMAL improves animal well-being and facilitates ethical approvals with painless and non-invasive sample collection.
- saliva-based collection methods are easy-to-use and reliable for use by pet owners or trained professionals in any collection environment: in the clinic, in the field or at home
- researchers can increase the number of samples collected and reduce collection cost and complexity by sending kits via the mail - no special shipping or handling is required
- study compliance can be increased and costly and inconvenient clinic visits required for a blood draw can be eliminated
Everyone at DNA Genotek congratulates Dr. Mitsouras on the publication of this research. A full case study on this research is also available.