DNA Genotek's Microbiome Collection and Stabilization Blog

Advances in microbiome discovery, diagnostics and therapeutics: Nature webcast highlights

Posted by Erin Carpenter on Aug 9, 2018 10:26:00 AM

Those of us following microbiome science know that it is developing extremely fast with tremendous opportunity for innovation. When we were presented the opportunity to participate in the recent webcast titled ‘Advances in Microbiome Discovery, Diagnostics and Therapeutics’ hosted by Nature.com, we were thrilled to agree. In this webinar, DNA Genotek and a panel of experts discussed their perspectives on advances in microbiome discovery. This webinar is available in its entirety on-demand (here), but knowing how time-pressed we all are, we have provided a summary for you below.

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Tags: microbiome, OMNIgene, microbiome collection, gut microbiome research

Choosing the best DNA extraction method in human gut microbiome community profiling

Posted by Erin Carpenter on Jul 30, 2018 9:06:00 AM

A critical component to any human gut microbiome study is the ability to accurately represent the ‘true’ microbial community present at a specific period in time or, in other words, a snapshot. With such a diverse sample type, there is considerable inter- and intra-subject variability, as well as methodological and technical sources of variation that can increase noise and obscure results. In this blog, we focus on discussing a technical source of variation – nucleic acid extraction methods. Read More

Tags: DNA extraction, omnigene gut, gut microbiome research

Microbiome highlights from Digestive Disease Week 2018

Posted by Erin Carpenter on Jul 5, 2018 9:51:00 AM

Digestive Disease Week 2018 (DDW2018) was another successful conference with over 450 sessions presented by invited speakers and abstract authors. The interest in how the microbiome could potentially impact digestive diseases grew even more this year as compared to past conferences, with more talks dedicated to the microbiome. Many of the talks were so popular they required overflow space in the hallways to accommodate all the interested audience members. You could feel the energy of new ideas being uncovered and knowledge being shared, and I felt privileged to be able to take part this year with a few of my fellow DNA Genotek colleagues.

With so many thought-provoking sessions to choose from, I certainly was not able to attend them all. With a particular interest in the gut microbiome, I wanted to share some of my highlights.

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Tags: microbiome collection, omnigene gut, gut microbiome research

Designing large-scale gut microbiome studies - 10 essential considerations

Posted by Erin Carpenter on Jun 12, 2018 9:51:00 AM

With a growing number of research studies showing associations between the gut microbiome and disease, this is a burgeoning field with fundamental questions that still need to be understood. The gut microbiome is composed of the community of microorganisms that dwell in our intestinal system, and elucidating the significance of this rich and diverse ecosystem is of great importance to human health. Large-scale studies with the sheer statistical power and immense volumes of data able to show strong correlations and draw meaningful conclusions are needed in this booming area of discovery.

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Tags: OMNIgene, microbiome stabilization, microbiome collection, gut microbiome research

DNA Genotek and the Mosaic Community Challenge: Standards

Posted by Aaron Del Duca on May 29, 2018 2:46:42 PM

If you are a frequent reader of The Microbiome Snapshot, you know that research into the human microbiome has emerged as highly informative to our overall understanding of health and disease risk. The growth in microbiome research has been fueled by many factors including the improvement of sequencing technologies and new computational methods. However, the impact of these methods have been somewhat limited by various factors such as precision, resolution, scalability and usability.

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Tags: gut microbiome, omnigene gut, gut microbiome research, microbiome standards

OMNIgene·GUT facilitating gut microbial collections in remote Himalayan populations

Posted by Erin Carpenter on Apr 6, 2018 9:20:00 AM

There is so much that can be learned from studying the gut microbiome. The community of bacteria co-habiting with their human host share a curious symbiotic relationship, one that can have a significant affect on health. This is a complex relationship where the abundance and diversity of the microbiome can affect various aspects of human physiology, metabolism, immune response, and psychology. Conversely, many factors within the human host’s control can alter the microbial community, even causing extinction events, such as dietary changes, geographical location, lifestyle, or antibiotic use. When this delicate balance is shifted, this may result in consequences for the host’s health. Therefore, it is essential to characterize the human microbiome and understand what factors alter this complex, reciprocal relationship.

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Tags: microbiome collection, omnigene gut, gut microbiome research

Harvard T.H. Chan School of Public Health selects DNA Genotek’s OMNIgene devices for Microbiome Biobank

Posted by Shauna White on Mar 21, 2018 8:38:42 AM

DNA Genotek Inc. is pleased to announce that the Harvard T.H. Chan School of Public Health has selected DNA Genotek’s OMNIgene® family of microbiome collection devices to provide reliable self-collection of microbial samples for The Biobank for Microbiome Research in Massachusetts (BIOM-Mass). DNA Genotek will also provide customization, fulfillment and logistics services through its GenoFIND services offering.

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Tags: oral microbiome, OMNIgene, microbiome collection, gut microbiome, gut microbiome research

Progressing gut microbiome research in IBD, one sample at a time

Posted by Seb Deneault on Dec 5, 2017 9:52:52 AM

Crohn’s and Colitis Awareness week was developed to raise awareness and increase understanding of these common diseases. Crohn’s disease and Ulcerative Colitis, together referred to as Inflammatory Bowel Disease, are serious illnesses that affect more than 5 million people worldwide. We would like to shed some light on these diseases, the treatments, and the interactions between the microbiome and IBD.

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Tags: OMNIgene, gut microbiome, gut microbiome research

Large population study highlights successful stool collection for microbiome analyses

Posted by Lisa Gamwell on Sep 21, 2017 10:03:19 AM

The human microbiome is one of the hottest topics of interest in the scientific community. The microbes in and on a healthy human adult are estimated to at least equal the number of human cells[1], which highlights the need for the scientific and medical community to better understand the broader impact on health and susceptibility to certain diseases/conditions and chronic illnesses. In particular, the gastrointestinal microbiome has captured the attention of researchers with stool specimens offering the most accessible means of assessing the gut microbial community[2]. However, there are few publications that focus on methods for collecting stool samples for large, population-based studies.

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Tags: microbiome, gut microbiome, omnigene gut, gut microbiome research

Sequencing the microbiome: Are you getting the full story?

Posted by Seb Deneault on Aug 14, 2017 2:31:00 PM

Evolution of sequencing technology

Since the human genome was sequenced and famously published in Nature and Science in 2001(1)(2), sequencing technology has experienced significant advancement. In 2005 several high- throughput approaches, collectively referred to as Next Generation Sequencing (NGS), quickly became preferable for larger projects compared to the more traditional Sanger sequencing, a capillary electrophoresis-based method used widely for close to 40 years. NGS technology produces many very short overlapping reads simultaneously, using massively parallel sequencing technology, such that each section of DNA (or RNA) is sequenced multiple times for exceptionally high coverage. The introduction of NGS played a huge role in revolutionizing the genomics field by lowering the cost of genome sequencing and by providing results 100 times faster than the Sanger approach. In 2010, the emergence of Third-Generation Sequencing (TGS) - enabled sequencing from a single molecule of DNA, thus eliminating the need for amplification and reducing PCR-derived bias. TGS produces substantially longer reads at 10K-15K base pairs compared to 100-600 base pairs for NGS, resulting in increased quality of genome assemblies through higher consensus accuracy and more uniform sequence coverage. For perspective on the evolution of sequencing, the 13-year-long, $2.7-billion international project (Human Genome Project) that sequenced the first human genome back in 2001, today would take little more than a day and would cost around $1,500 using NGS.

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Tags: sequencing, microbiome collection, gut microbiome, gut microbiome research

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This blog is intended to provide information to educate readers about microbiome sample collection, stabilization and DNA Genotek products. Some of the information on this blog represents emerging scientific research or data developed for research purposes only. More information here.

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About DNA Genotek

Welcome to The Microbiome Snapshot, a blog providing new insights into microbiome sample collection and stabilization by DNA Genotek. DNA Genotek Inc. is a subsidiary of OraSure Technologies, Inc.

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