DNA Genotek's Microbiome Collection and Stabilization Blog

Are these popular diets the best solution to reduce IBD and gut microbial dysbiosis?

Posted by Katherine Lawless on May 19, 2019 9:44:00 AM

What should I eat? - One of the most common questions asked by Inflammatory Bowel Disease (IBD) patients. The role of diet has become increasingly important in the prevention and treatment of IBD.[1] In recent research, changes in the human gut microbiome have been detected in different forms of IBD such as Crohn’s disease (CD) and ulcerative colitis (UC), supporting the hypothesis of dysbiosis as a relevant component to IBD pathogenesis.[2] Researchers discovered that the gut microbiome of African children were rich in Bacteriodetes and poor in firmicutes and Enterobacteriaceae, while the results obtained from European children was the opposite due to differences in their diet. 3

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Tags: gut microbiome, omnigene gut, gut microbiome research, IBD

Cold chain stool sample collection: A series of unfortunate events (part 2/3)

Posted by Zara Jennings on May 1, 2019 11:23:00 AM

Most people consider the nature of collecting fecal samples unpleasant and inconvenient. One of the challenges for the researcher conducting a gut microbiome study, is to provide the participants with a comprehensive and simple collection procedure without compromising the integrity of the sample. There is a mountain of data on collection methods, temperature holds, stabilizers vs fresh, individual chemistries, effects of freezing on viability, and DNA integrity…how can we make sense out of this often conflicting data?

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Tags: OMNIgene, omnigene gut, microbiome standards, microbiome sample collection

Cold chain stool sample collection: a series of unfortunate events (Part 1/3)

Posted by Ali Acar on Apr 9, 2019 1:22:00 PM

In this first of a three-part blog series, I am delving into the topic of cold-chain stool collection and transportation. I will explore the challenges of collection and transportation using cold-chain in part 1, take a deep dive into the potential sources of error and bias introduced in part 2, and lastly outline best practices for performing validations in part 3.

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Tags: gut microbiome, omnigene gut, gut microbiome research, microbiome sample collection

The importance of the gut microbiome in predicting dietary responses

Posted by Fatima Mahmood on Apr 2, 2019 1:34:00 PM

Personalized nutrition is emerging as a field of interest due to an increase in coverage in the general media and the growing awareness that no two people can have identical responses to the same meal. One research paper that detailed the unique postprandial glycemic response (PPGR) to dietary intake was published by Dr. Segal and Dr. Elinav at the Weizmann Institute in 2015. In the past, this variable response from person to person has been identified however the specific factors behind this variability are described for the first time in this paper. Currently, to achieve a homeostatic glycemic balance, carbohydrate intake is primarily taken in to consideration. The Segal lab took this a step further and created an algorithm based on blood parameters, anthropometrics, physical activity, lifestyle behaviours and the gut microbiome which allows for individual predictions of PPGRs in response to different foods.  

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Tags: gut microbiome, gut microbiome research, personalized nutrition

A look ahead for the microbiome industry: An interview with Dr. Dan Knights

Posted by Shauna White on Mar 27, 2019 2:01:00 PM

We recently introduced readers of The Microbiome Snapshot to our sister company, CoreBiome, a privately-held, Minnesota-based microbiome analysis company that accelerates discovery for customers in the pharmaceutical, agricultural, environmental and research communities. We are continuing this story via a podcast with CoreBiome co-founder, Dr. Dan Knights. In this new podcast, Dr. Knights speaks to us about why the microbiome is so different from the human genome, what answers microbiome research can provide for wellness and disease that we can’t find elsewhere, and what advice he would give an epidemiologist who is looking to add a microbiome component to their project.

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Tags: microbiome sample collection, microbiome extraction, microbiome analysis, shotgun sequencing

Inspirational women in the microbiome field - International women's day

Posted by Katherine Lawless on Mar 8, 2019 8:32:00 AM

Throughout history women have influenced our views and knowledge of science from all disciplines, including microbiology. Through the co-development of an antitoxin for diphtheria by Dr. Anna Wessel Williams (1863-1954), grouping and typing of streptococci by Dr. Rebecca Craighill Lancefield (1895-1981), and the research on Haemophilus influenzae and Bordetella pertussis by a woman hailed as "woman scientist ahead of her time," Dr. Margaret Pittman (1902-1995), women have  influenced the study of microbiology.[1] The microbiome, a subset of microbiology, is a newer field and is rapidly advancing. Many of these advancements are being led by female scientists from all over the world.  

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Tags: gut microbiome research, microbiome analysis

Enabling big data and good data at the same time: The microbiome challenges CoreBiome was built to solve

Posted by Shauna White on Mar 4, 2019 1:24:00 PM

As a reader of The Microbiome Snapshot, you are likely aware that we recently acquired CoreBiome, a privately-held, Minnesota-based microbiome services provider that accelerates discovery for customers in the pharmaceutical, agricultural, and research communities. Many of you may also be aware of the strong reputation CoreBiome has as a leading service provider in the microbiome field – specifically for reducing costs while maximizing output for microbiome discovery.

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Tags: microbiome sample collection, microbiome extraction, microbiome analysis, shotgun sequencing

Why is an optimal DNA extraction important for microbial profiling?

Posted by Fatima Mahmood on Feb 12, 2019 1:31:00 PM

One of the many ways to ensure optimal isolation of bacterial DNA from stool samples, is through the use of an efficient extraction methodology. Our customers have been asking for extraction recommendations, so we conducted a validation study comparing four different manual extraction methods. There are many steps during the DNA isolation process where a multitude of biases can be introduced. The OMNIgene·GUT device reduces biases during stool sample collection to provide our users with an accurate snapshot of the in vivo gut microbial DNA content at the time of collection. This snapshot is maintained at ambient temperature for 60 days making it flexible and reliable to use when you are ready to extract microbial DNA.

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Tags: gut microbiome, omnigene gut, microbiome extraction

DNA Genotek's top 3 gut microbiome publication picks for 2018

Posted by Erin Carpenter on Dec 13, 2018 10:05:00 AM

It is no secret that gut microbiome research has been on the rise leading to a plethora of new insights, theories and discoveries. Like you – we’ve been absolutely captivated watching this burgeoning field evolve and introduce many new areas of advancement in theoretical understanding as well as direct therapeutic applications. There was certainly no shortage of exciting new publications in the gut microbiome field this year, and it was a challenge to narrow down our list – but we would like to share what we chose as our top three gut microbiome publication selections from 2018 with you.

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Tags: gut microbiome, omnigene gut, gut microbiome research

Collect and stabilize gut microbiota samples from IBD patients undergoing dysbiosis

Posted by Erin Carpenter on Nov 6, 2018 1:26:42 PM

In human gut microbiome studies, the logistics of immediately freezing and transporting collected stool samples on dry ice is not always feasible - especially with at-home collections. If the samples are not immediately stabilized, taxonomic profile changes can occur rapidly at room temperature. Furthermore, a challenge with collecting samples from patients exhibiting symptoms of inflammatory bowel disease (IBD) or Crohn’s and chronic colitis, is stool samples can be liquid (Bristol stool type 6 and 7), complicating collection and sample handling, as well as causing a barrier to donor enrolment. To facilitate large cohort studies and produce high quality data, researchers need to be equipped with the most effective tools to easily collect, stabilize and store precious samples.

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Tags: feces collection, gut microbiome research, microbiome sample collection

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This blog is intended to provide information to educate readers about microbiome sample collection, stabilization and DNA Genotek products. Some of the information on this blog represents emerging scientific research or data developed for research purposes only. More information here.

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About DNA Genotek

Welcome to The Microbiome Snapshot, a blog providing new insights into microbiome sample collection and stabilization by DNA Genotek. DNA Genotek Inc. is a subsidiary of OraSure Technologies, Inc.

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