The Microbiome Snapshot

a blog providing new insights into Microbiome Collection and Stabilization by DNA Genotek. DNA Genotek is a subsidiary of OraSure Technologies, Inc.

All Posts

Article by: Lisa Gamwell

The Personalized Nutrition Project: An in-depth look at sample collection and stabilization


personalized_nutrition_blog_picture.jpgYou’ve likely heard about the Weizmann Institute’s Personalized Nutrition Project.  The findings of this research study, which were published on November 19th, 2015 by the research groups of Eran Elinav and Eran Segal in the journal Cell, have garnered a lot of attention from the media.  Their study, entitled “Personalized Nutrition by Prediction of Glycemic Responses” suggest that there is no “one-size-fits-all” diet.  For some people, eating sushi will raise their blood sugar more than eating ice cream, while for others it is the opposite.

We recently interviewed one of the authors, Dr. Adina Weinberger, and discussed the intricacies of collecting and stabilizing gut microbiome samples from their 800-person cohort.  Dr. Weinberger considers the collection stage of any study workflow a critical success factor, and she and the rest of her collaborators have taken great strides to standardize their gut microbiome collection protocols. In their current publication they used swabs and/or OMNIgene•GUT to collect gut microbiome samples from hundreds of healthy individuals. They reported that when comparing a frozen swab to a sample collected with OMNIgene•GUT,

“the relative abundances for the same person are highly correlated (R=0.99 P< 10-10)”.

In our interview with Dr. Weinberger, she elaborates on the two collection methods used in this publication, “before we started using DNA Genotek’s collection and stabilization kit (OMNIgene●GUT) we used swabs which was standard procedure in this field. We noticed that when we used swabs, although we were very strict in our collection procedure, providing coolers to our users and freezing the samples immediately in -80°C freezers, we noticed in some of the swabs there was some overgrowth of bacteria on the swab after collection.”

You can learn more about how they tackled the challenge of collecting and stabilizing gut microbiome samples for 800+ healthy individuals and the steps they took to make their lab protocols 30% more efficient in our blog post Microbiome learnings from the Weizmann Institute, first published on May 26, 2015.

Have you experienced similar challenges in your cohort? What’s your favourite finding from the Personalized Nutrition Project? 

New Call-to-action

Image source: Zeevi et al. Cell. 2015 Nov 19;163(5):1079-94.


Subscribe and Get The Latest News

Input your email to subscribe

Recent Posts

OMNIgene™•ORAL and OMNIgene™•VAGINAL devices: validated tools for the collection and stabilization of nucleic acids for microbiome sampling

The oral and vaginal cavities of the human body each contain a distinct collection of microbial communiti...
Sanna Abbasi, PhD Aug 9, 2023 10:10:01 AM

Reviewing current challenges and advances in skin microbiome research

Over the past several years, the skin microbiome has emerged as an important research field of interest f...
Sanna Abbasi, PhD Jun 14, 2023 1:06:12 PM

Three reasons why the OMNIgene™•GUT all-in-one system is ideal for pediatric gut microbiome profiling

In a recently published white paper, DNA Genotek researchers utilized the OMNIgene•GUT system (OM-200 and...
Sanna Abbasi, PhD Nov 29, 2022 4:03:48 PM